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Genetic Complementation and Recombination Spot Test Kit (with Prepaid Coupon)

$252.35
Product: (in stock)

Description

All materials and instructions for (1) conducting complementation (cis-trans) spot tests to place unknown T4rll mutations in either cistron A or B, and for (2) conducting recombination spot tests to determine by deletion mapping the A-segments within which the rll mutations occur. Exercises are recommended for an individual, a team of 2 or 3 students, or use as a demonstration. Other students, however, can participate when they are given sets of result plates for personal analysis and conclusions. Results are obtained from 4 spot tests and 2 control plates. During the course of the experimental procedure described, up to 8 additional sets of 6 plates each can be easily prepared for distribution to other students. Kit provides enough phage and bacteria for such duplication of results, but the required agar plates and soft agar must be purchased separately. Includes a prepaid coupon to request perishable materials at a later date. Note: Please refer to Bacteriophage Media and item #124730 Plaque Assay Plating Set. Refrigerate coliphage until use; do not freeze. Keep bacteria culture and agar at room temperature.

For Educational Use Only. All orders are subject to Carolina’s Terms and Conditions

Specifications

What’s Included:
  • 1 1 x 108 T4rllA Known Mutant Tester, 3 mL
  • 1 1 x 109 T4rllAB Reference Deletion Mutant X, 3 mL
  • 1 1 x 109 T4rllAB Reference Deletion Mutant Y, 3 mL
  • 1 1 x 109 T4rllAB Reference Deletion Mutant Z, 3 mL
  • 1 1 x 109 T4rll Unknown Mutant No. 1, 5 mL
  • 1 1 x 109 T4rll Unknown Mutant No. 2, 5 mL
  • 1 1 x 109 T4rll Unknown Mutant No. 3, 5 mL
  • 1 E. coli B, Agar Slant Culture
  • 1 E. coli K12 (lambda), Agar Slant Culture
  • 1 Nutrient Peptone Broth, 80 mL
  • 2 Base Layer Agar, 125 mL
  • 20 Sterile Petri Dishes
  • 1 Dropper Vial, 5 mL
  • 21 Glass Spotting Rods, 150 mm
  • 3 Sterile Disposable Dropping Pipets
  • 6 Screw-cap Vials (labeled for genetic crosses)
  • 35 Transfer Pipets, 1 mL
  • 3 Bulb, 1 cc
  • 3 Tubes Broth, 5 mL
  • 4 Tubes Broth for 10-Fold Dilutions, 9 mL
  • 13 Tubes Broth for 100-Fold Dilutions, 9.9 mL
  • 10 Soft Agar, 2 mL
  • 1 Autoclave Disposable Bag
  • 1 Wax Marker
  • 10 Procedure Flow Sheets
  • 10 Instruction Manuals with Worksheets
  • expand to see full list
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