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AdvancedFor experienced high school and college classes; requires some technical skill.
This biology lab explores the engineering of a yeast that's genetically modified to produce ß-carotene. In the body, ß-carotene is converted to vitamin A, which is crucial for vision, the immune system, and other biological functions. Use PCR to test the metabolic pathway of modified Saccharomyces cerevisiae as part of the engineering design-build-test pathway and investigate how to make it possible for users to bake vitamin A-enriched loaves of bread. Kit includes enough materials for 24 students working in 6 groups.
Students will perform:
This lab may be performed on its own or in conjunction with the BioBuilder® Golden Bread Transformation Kit (items #217006 and #217006P). PCR could be used to test for gene content of different colored colonies before and after transformation. The transformation kit inserts a second copy of one of the enzymes needed in the biosynthetic pathway of ß-carotene, scientifically addressing the importance of genetic redundancy when engineering synthetic systems.
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