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Intermediate—Easy to perform; requires some background knowledge.
In this lab, students use a fluorescent dye to investigate the conditions that influence DNA structure and its transition from double helix to single strand, and vice versa. Students can test conditions that influence Watson-Crick base pairing between complementary DNA strands, using temperature to differentiate between DNA of different sequences, and establishing the melting temperature of each sample. They carry out 3 investigations exploring DNA composition, concentration, the chemical milieu, and their influence on Watson-Crick pairing. Kit includes sufficient materials for a class of 32 working in 8 groups. Note: Ships with perishable materials. Keep refrigerated until use.
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