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Intermediate - Easy to perform; requires some basic training in microbiology. For a class of 30 students or for 6 separate teacher demonstrations. Demonstrates the extraction of DNA from M. luteus cells. Cell walls are broken with a detergent, and the DNA is extracted onto a spooling rod. The exercise helps students visualize DNA and understand some of its properties. Requires 1 to 2 class periods to complete.
Intermediate - Easy to perform; requires some basic training in microbiology.
For a class of 30 students or for 6 separate teacher demonstrations. Demonstrates the extraction of DNA from M. luteus cells. Cell walls are broken with a detergent, and the DNA is extracted onto a spooling rod. The exercise helps students visualize DNA and understand some of its properties. A hot water bath and 70 to 95% ethanol are needed but not included. Requires 1 to 2 class periods to complete. Once the lysozyme has been activated, it must be used immediately to ensure successful extraction of DNA from the M. luteus.
The Refill contains the perishable materials needed to rerun the classroom kit. With instructions.
