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Intermediate - Easy to perform; requires some basic training in microbiology.
For a class of 30 students or for 6 separate teacher demonstrations. Demonstrates the extraction of DNA from M. luteus cells. Cell walls are broken with a detergent, and the DNA is extracted onto a spooling rod. The exercise helps students visualize DNA and understand some of its properties. A hot water bath and 70 to 95% ethanol are needed but not included. Requires 1 to 2 class periods to complete. Once the lysozyme has been activated, it must be used immediately to ensure successful extraction of DNA from the M. luteus. With instructions.
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