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Inducing RNAi by Feeding Kit (with perishables)

$201.75
Product: (backordered: in stock 4/28/22)

Description

Advanced – For experienced high school and college classes; requires some technical skill.

  • Demonstrate to your students the power of silencing a single gene.
  • Teach about a powerful method for determining gene function.
  • Introduce your students to a model organism used for studying many aspects of biology, including development and gene function.
  • Engage in bioinformatics exercises exploring protein function and C. elegans and human gene relatedness.
In this lab, wild-type worms are fed lab strains of E. coli that express double-stranded RNA (dsRNA) corresponding to either of 2 target genes: BLIstered cuticle (bli-1) or DumPY (dpy-11). The ingestion of the dsRNA initiates the destruction of mRNA expressed from the target genes. Students then examine the phenotypes induced by silencing these genes. Silencing bli-1 leads to the formation of clear blisters in a worm's outer cuticle; silencing dpy-11 leads to a worm developing a short, fat body. In accompanying bioinformatics exercises, students explore the function of the protein encoded by the silenced gene and the relatedness of worm and human genes.

Note: Because of the time-sensitive preparation and limited shelf life of the kit's perishable materials, please order a minimum of 2 weeks prior to the desired ship date. Plan on using the materials within a week of receiving them. Perishables ship only on Mondays, Tuesdays, and Wednesdays via standard overnight delivery.

FREE 1-year access to digital resources that support NGSS instruction included.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This is a special order item that requires additional time for culturing and processing before it ships. Arrival timeframes are available during Checkout.
What’s Included:
  • 2 Cholesterol, 5 mg/mL, 0.3 mL  
  • 1 IPTG, 0.036 g  
  • 2 Wild-type (N2) C. elegans  
  • 1 OP50 E. coli Slant Culture  
  • 1 RNA Feeding Strain BLI-1, Slant Culture  
  • 1 RNA Feeding Strain DPY-11, Slant Culture  
  • 1 Ampicillin, 10 mg/mL, 4 mL  
  • 2 NGM-lite, 300 mL
  • 12 Luria Broth, 3 mL
  • 1 Sterile Distilled Water, 1 mL
  • 24 Sterile Culture Tubes
  • 28 Inoculating Loops
  • 28 Pipets, 1 mL
  • 8 Sterile Toothpicks
  • 40 Sterile Petri Dishes
  • 1 Teacher's Manual and Reproducible Student Guide
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Needed But Not Included:
  •  Document Camera or Overhead Projector
  • 1 Heavy Duty Trash Bag
  •  Bleach or Disinfectant
  •  Stereomicroscope with Transillumination
  •  Bunsen Burner
  •  Ethanol, 95%
  •  Micropipettors and Tips (30 µL, 120 µL, 150 µL)
  •  Permanent Markers, Black and Red
  •  Shaking Water Bath or Incubator
  •  Test Tube Rack
  •  Microwave Oven
  •  Steel Spatulas (for chunking worms)
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Return Policy:

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