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Examining the RNAi Mechanism Kit (RNAi and Amplification; with Prepaid Coupon)

$317.30
Product: (in stock)

Description

Advanced - For experienced high school and college classes; requires some basic technical skill.

  • Demonstrate to your students the power of silencing a single gene
  • Teach about a powerful method for determining gene function
  • Explore the relationship between phenotype, genotype, and RNAi-induced phenotype
  • Perform bioinformatics exercises exploring protein function and C. elegans, and human relatedness
RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading the gene's mRNA. Examining the RNAi Mechanism kit allows students to use this Nobel Prize-winning technique to silence the dpy-13 gene in the nonparasitic roundworm C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, dumpy 13 (dpy-13).

Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishables.

FREE 1-year access to digital resources that support NGSS instruction included.

Specifications

What’s Included:
  • 1 Ampicillin, 10 mg/mL, 4 mL
  • 2 Cholesterol, 5 mg/mL, 0.3 mL
  • 1 IPTG, 0.036 g
  • 1 Proteinase K, 20 mg/mL, 25 μL
  • 1 DNA Marker pBR322/BstN1, 200 μL
  • dpy-13 (e458) Worms on NGM-lite Plates
  • dpy-13 Primer/Loading Dye Mix, 700 μL
  • E. coli RNA Feeding Strain (dpy-13) LB Amp/Tet Slant
  • E. coli OP50 on LB Slant
  • 4 Wild-type Worms on NGM-lite Plates
  • 1 1x Lysis Buffer, 0.5 mL
  • 6 Sterile Bulb Pipets
  • 3 Sterile Culture Tubes, 15 mL
  • 3 Sterile Inoculating Loops
  • 3 LB Broth, 3 mL
  • 1 Mineral Oil, 5 mL
  • 1 Melt 'n Pour NGM-lite, 300 mL
  • 1 Melt 'n Pour NGM-lite, 100 mL
  • 40 Sterile Petri Dishes, 60 mm
  • 25 Ready-to-Go™ PCR Beads (0.2 mL tubes)
  • 1 Sterile Distilled Water, 1 mL
  • 8 Sterile Toothpicks
  • 1 Teacher's Manual and Reproducible Student Guide
  • expand to see full list
Needed But Not Included:
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