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Beginning—Easy to perform; requires no experience in microbiology.
For a class of 30 students. Members of the genus Rhizobium exist in a symbiotic relationship with leguminous plants (peas, clover, and soybeans). The Rhizobium growing in nodules on the roots of leguminous plants fix atmospheric nitrogen (N2). This supplies plants with nitrates and bacteria with energy and a favorable environment. In farming, legumes are plowed under, adding significant amounts of N2 to the soil. Nodules can be observed 6 weeks after planting. Note: This kit includes perishable materials. Refrigerate inoculum and seed until use; do not freeze.
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